Identification of the heat shock protein 60 epitope involved in receptor binding on macrophages
- 18 May 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 568 (1-3), 65-69
- https://doi.org/10.1016/j.febslet.2004.05.010
Abstract
In the present study, we identified the human heat shock protein 60 (HSP60) epitope responsible for binding to macrophages. Studies using overlapping 15- and 20-mer peptides of the human HSP60 sequence to compete with binding of HSP60 to macrophages indicated that surface binding was accounted for by the region aa481-500. Deletion mutants of HSP60, lacking the N-terminal 137, 243 or 359 amino acids, strongly inhibited HSP60 binding to macrophages. Monoclonal antibodies addressing regions aa1-200, aa335-366 or aa383-447 did not block HSP60 binding. We conclude that a single C-terminal region, aa481-500, accounts for the binding of HSP60 to macrophages.Keywords
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