Physical and functional heterogeneity in TYMV RNA : evidence for the existence of an independent messenger coding for coat protein
- 1 November 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (11), 3043-3062
- https://doi.org/10.1093/nar/3.11.3043
Abstract
Turnip yellow mosaic virus RNA can be separated into two distinct components of 2 × 106 and 300 000 daltons molecular weight after moderate heat treatment in the presence of SDS or EDTA. The two species cannot have arisen by accidental in vitro degradation of a larger RNA, as they both possess capped 5′ ends. Analysis of the newly synthesized proteins resulting from translation of each RNA by a wheat germ extract shows that the 300 000 molecular weight RNA can be translated very efficiently into coat protein. When translated in vitro the longer RNA gave a series of high molecular weight polypeptides but only very small amounts of a polypeptide having about the same mass as the coat protein. Thus our results suggest that the small RNA is the functional messenger for coat protein synthesis in infected cells.Keywords
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