ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES TO PNEUMONIA VIRUS OF MICE IN RAT SERA
- 1 January 1978
- journal article
- research article
- Vol. 28 (6), 676-679
Abstract
An enzyme linked immunosorbent assay (ELISA) was established to detect rat serum antibodies [Ab] to pneumonia virus of mice (PVM). Vero [African green monkey kidney] cells infected with the virus were adsorbed to the surface of microplates, and the presence of Ab in the sample evaluated was demonstrated by the subsequent use of goat anti-rat immunoglobulin G conjugated to peroxidase and by specific substrate. Ab against pneumonia virus of mice might be detected in rat serum with the ELISA procedure. The results were reproducible, and the method was approximately 8 times more sensitive than the hemagglutination inhibition procedure.This publication has 5 references indexed in Scilit:
- Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infectionInfection and Immunity, 1978
- Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirusJournal of Clinical Microbiology, 1977
- DEVELOPMENT AND EVALUATION OF AN ENZYME-LABELED ANTIBODY-TEST FOR RAPID DETECTION OF HOG-CHOLERA ANTIBODIES1977
- Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody testJournal of Clinical Microbiology, 1976
- Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigsJournal of Immunological Methods, 1976