Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution

Abstract

Spectrally resolved emission (270–560 nm) from dilute suspensions of washed Bacillus subtilis and Escherichia coli were measured by use of tunable laser excitation between 270 and 300 nm. Integrated absolute emission cross sections increase with decreasing excitation wavelength and range from 1.8 × 10^-12 to 6.0 × 10^-11 cm²/(particle sr). An emission band near 340 nm dominates all observed spectra. At each excitation wavelength spectrally resolved emissions from the E. coli and B. subtilis suspensions are indistinguishable.