Characterization of the 3′ Termini of the RNAs of Cowpea Mosaic Virus
- 1 May 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 26 (2), 243-248
- https://doi.org/10.1128/jvi.26.2.243-248.1978
Abstract
A sequence of poly(A), homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNA of cowpea mosaic virus. The poly(A) was 3''-terminal and occurred once per molecule. A fragment consisting of the poly(A) and approximately the next 25 nucleotides could be isolated from complete T1 RNase digests of either RNA. The region adjacent to the poly(A) in both RNA was rich in pyrimidines. The mobilities of the fragments in 12.4% polyacrylamide-8 M urea gels were used to estimate their lengths and to calculate number average and weight average MW.This publication has 14 references indexed in Scilit:
- Biological and physicochemical characteristics of three strains of red clover mottle virusVirology, 1976
- Similarity in structure and function of the 3′-terminal region of the four brome mosaic viral RNAsJournal of Molecular Biology, 1976
- Detection of polyadenylic acid sequences in plant pathogenic RNAsVirology, 1974
- Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditionsVirology, 1974
- [56] The isolation of messenger RNA from mammalian cellsMethods in Enzymology, 1974
- Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virusVirology, 1973
- The localization of virus-specific double-stranded RNA of cowpea mosaic virus in subcellular fractions of infected Vigna leavesVirology, 1973
- The 3′-termini of bacteriophage Qβ plus and minus strandsJournal of Molecular Biology, 1970
- Analysis of nucleic acid derivatives at the subnanomole levelAnalytical Biochemistry, 1969
- Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis*Biochemistry, 1967