Polyphosphate enhances fibrin clot structure
- 1 October 2008
- journal article
- Published by American Society of Hematology in Blood
- Vol. 112 (7), 2810-2816
- https://doi.org/10.1182/blood-2008-03-145755
Abstract
Polyphosphate, a linear polymer of inorganic phosphate, is present in platelet dense granules and is secreted on platelet activation. We recently reported that polyphosphate is a potent hemostatic regulator, serving to activate the contact pathway of blood clotting and accelerate factor V activation. Because polyphosphate did not alter thrombin clotting times, it appeared to exert all its procoagulant actions upstream of thrombin. We now report that polyphosphate enhances fibrin clot structure in a calcium-dependent manner. Fibrin clots formed in the presence of polyphosphate had up to 3-fold higher turbidity, had higher mass-length ratios, and exhibited thicker fibers in scanning electron micrographs. The ability of polyphosphate to enhance fibrin clot turbidity was independent of factor XIIIa activity. When plasmin or a combination of plasminogen and tissue plasminogen activators were included in clotting reactions, fibrin clots formed in the presence of polyphosphate exhibited prolonged clot lysis times. Release of polyphosphate from activated platelets or infectious microorganisms may play an important role in modulating fibrin clot structure and increasing its resistance to fibrinolysis. Polyphosphate may also be useful in enhancing the structure of surgical fibrin sealants.Keywords
This publication has 46 references indexed in Scilit:
- Polyphosphate blocks tumour metastasis via anti-angiogenic activityBiochemical Journal, 2007
- Thrombin generation and fibrin clot structureBlood Reviews, 2007
- Applications of Fibrin Sealant in SurgerySurgical Innovation, 2005
- Kinetics of Fibrinopeptide Release by Thrombin as a Function of CaCl2 Concentration: Different Susceptibility of FPA and FPB and Evidence for a Fibrinogen Isoform-Specific Effect at Physiological Ca2+ ConcentrationBiochemistry, 2003
- Fibrinogen gamma-chain splice variant γ′ alters fibrin formation and structureBlood, 2003
- Interaction of Fibrin(ogen) with Heparin: Further Characterization and Localization of the Heparin-Binding SiteBiochemistry, 2003
- The Ultrastructure of Fibrinogen Caracas II Molecules, Fibers, and ClotsPublished by Elsevier ,1996
- Effect of fibrin structure on plasmin-mediated dissolution of plasma clotsBlood Coagulation & Fibrinolysis, 1995
- Effects of low molecular weight heparins on fibrin polymerization and clot sensitivity to t-PA-induced lysisBlood Coagulation & Fibrinolysis, 1993
- Fibrin clots obtained from plasma containing heparin show a higher sensitivity to t-PA-induced lysisBlood Coagulation & Fibrinolysis, 1992