Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the texas male sterile cytoplasm
- 31 August 1982
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 62 (3), 213-217
- https://doi.org/10.1007/bf00276241
Abstract
Summary Maize plants carrying Texas (T) cytoplasm are male-sterile and sensitive to Drechslera maydis race T toxin, whereas plants carrying Normal (N) cytoplasm are male-fertile and resistant to the toxin. Some plants regenerated from T cytoplasm tissue cultures exhibit a N cytoplasm-like phenotype with respect to malefertility and toxin-resistance. Analysis of the high molecular weight mitochondrial DNA (mtDNA) of such regenerants has shown that the plants do not contain N cytoplasm mtDNA. However, their mtDNAs do show sequence differences from each other and from the mtDNA of plants possessing T cytoplasm. No single alteration detected correlates with the change to malefertility or toxin resistance. Sequence alterations were also evident in high molecular weight mtDNA isolated from a plant regenerated from N cytoplasm callus. No changes in low molecular weight mtDNA molecules were observed in regenerants from N or T cytoplasm callus.Keywords
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