• 1 January 1985
    • journal article
    • research article
    • Vol. 17 (1), 23-33
Abstract
1H NMR spectra of excised rat brain were recorded at 361 MHz using 2 different H2O suppression pulse sequences. The assignment of the resonances was carried out in perchloric acid extracts and subcellular fractions. Cytosolic proteins, membrane phospholipids and 16 different metabolities evidently contribute to the observed spectra. The new resonances assigned allow the direct observation of myo-inositol and urea. Changes in the spectral pattern upon anesthesia, ischemic exposure of the brain and age of the rat were recorded and correlated with the compounds producing the spectra.