Electron Spin Resonance for Detecting Polyadenylate Tracts in RNA's
- 11 April 1975
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 188 (4184), 153-155
- https://doi.org/10.1126/science.163495
Abstract
Electron spin resonance is used to detect RNA's that contain polyadenylate tracts. The method depends on the ability of RNA's that contain polyadenylate sequences to associate with poly(2'-deoxy-2'-fluoro)uridylic acid, which has been spin-labeled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl. The formation of the hybridization product can be detected by monitoring the decrease in mobility of the spin probe.Keywords
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