Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli
- 1 June 1975
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 255 (5509), 533-538
- https://doi.org/10.1038/255533a0
Abstract
Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.Keywords
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