Inhibition of adenylate cyclase by general anesthetics in toad urinary bladder
- 1 November 1979
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 237 (5), F372-F378
- https://doi.org/10.1152/ajprenal.1979.237.5.f372
Abstract
General anesthetic agents modulate the adenylate cyclase-cyclic[c]AMP system in several tissues. The anesthetics methohexital and methoxyflurane selectively inhibit vasopressin-stimulated water flow in the toad urinary bladder, leaving urea permeability unaltered. The site of action of the drugs was studied. Both anesthetics inhibited vasopressin-stimulated adenylate cyclase activity over a wide range of vasopressin concentrations. Fluoride-stimulated activities were unaffected by either anesthetic. Both anesthetics diminished the vasopressin-related increment in intracellular cAMP levels. Phosphodiesterase activities were unaltered. The long-acting barbiturate phenobarbital inhibited both vasopressin-stimulated water flow and adenylate cyclase. The concentration of phenobarbital required for both of the effects was 10-20 times that required for the more lipid-soluble barbiturate methohexital; apparently partition into membrane lipid may be essential for the barbiturate effects on vasopressin stimulation of both transport and adenylate cyclase. Apparently the anesthetics tested inhibit vasopressin-stimulated water flow by reducing stimulation of adenylate cyclase, primarily by interfering with the hormone-receptor or receptor-catalytic subunit interactions. Substantial vasopressin stimulation of water and urea permeabilities were demonstrated under conditions in which adenylate cyclase or cAMP level enhancement was not measured. Apparently the cAMP pool or pools related to control of water and urea permeabilities may be small compared to total cell cAMP content, or that some portion of vasopressin''s effect on water or urea or both may be mediated by a cAMP-independent mechanism.This publication has 10 references indexed in Scilit:
- Rapid assay for cyclic AMP and cyclic GMP phosphodiesterasesAnalytical Biochemistry, 1978
- Intermediate role of adenosine 3′:5′-cyclic monophosphate and protein kinase during gonadotropin-induced steroidogenesis in testicular interstitial cellsProceedings of the National Academy of Sciences, 1977
- ACTION OF HALOTHANE ON ADENYLATE-CYCLASE1977
- Selective inhibition of osmotic water flow by general anesthetics to toad urinary bladder.JCI Insight, 1976
- Ca++ and Mg++ effects on toad bladder response to cyclic AMP, theophylline, and ADH analoguesAmerican Journal of Physiology-Legacy Content, 1967
- ADENYL CYCLASE AS AN ADRENERGIC RECEPTOR*Annals of the New York Academy of Sciences, 1967
- Calcium Inhibition of the Action of Vasopressin on the Urinary Bladder of the Toad*JCI Insight, 1964
- THE EFFECT OF NEUROHYPOPHYSEAL HORMONES ON THE PERMEABILITY OF THE TOAD BLADDER TO UREA*JCI Insight, 1960
- THE EFFECTS OF NEUROHYPOPHYSIAL EXTRACTS ON WATER TRANSFER ACROSS THE WALL OF THE ISOLATED URINARY BLADDER OF THE TOAD BUFO MARINUSJournal of Endocrinology, 1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951