Comparison of Festuca Leaf Streak Virus Antigens with Those of Three Other Rhabdoviruses Infecting the Gramineae

Abstract

Leaf tissue from plants infected with Festuca leaf streak virus was extracted with a buffer containing a nonionic detergent; this treatment released the G protein from the nucleocapsid of virions. The extract was tested with antiserum by double immunodiffusion for G protein and by immunosorbent electron microscopy with ‘decoration’ for nucleocapsid antigens. Clear homologous reactions were obtained. No heterologous reactions were observed with similar preparations of barley yellow striate mosaic, northern cereal mosaic, and cereal chlorotic mottle viruses.