Erythropoietin activity in the serum of beta thalassemic patients
- 1 September 1986
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Haematology
- Vol. 37 (3), 221-228
- https://doi.org/10.1111/j.1600-0609.1986.tb02301.x
Abstract
Serum erythropoietic activity was determined in 32 patients with beta thalassemia major and intermedia. Quantitation was performed by an in vitro bioassay using rabbit erythroid precursor cells (CFU-E) either by colony assay or by 3H-thymidine uptake. 20 polytransfused beta-thalassemic major patients had erythropoietic activity (mean 89.3 .+-. 36 milliunits/ml) which was not significantly different (p > 0.2) from normal individuals (51.3 .+-. 32 milliunits/ml). 12 untransfused patients with beta thalassemia intermedia were found to have comparable serum erythropoietic activity (p > 0.1). These levels were much lower than those found in patients with aplastic anemia who had a comparable degree of anemia. We have shown that the low EPO activity in thalassemic patients was not due to experimental conditions (excess of ferritin, low transferrin) nor to specific inhibitors appearing in this disease. No correlation was found between the erythropoietic activity and sex or other clinical parameters of the patients such as severity of the anemia, splenectomy, iron chelation or transfusion therapy, 4 young thalassemia children (1-2 yr of age) studied had high erythropoietic activity ranging from 661 to 5793 milliunits/ml-significantly different from normal children of the same age. It is suggested, therefore, that a decrease in serum erythropoietin levels develops during the course of the disease.Keywords
This publication has 21 references indexed in Scilit:
- Relation between Erythropoiesis and Bone Metabolism in ThalassemiaNew England Journal of Medicine, 1981
- GLOBIN SYNTHESIS IN SEVERE AND INTERMEDIATE HOMOZYGOUS β THALASSEMIA IN ISRAEL *Annals of the New York Academy of Sciences, 1980
- Suppressive Effect of Ferritin on in Vitro Lymphocyte FunctionBritish Journal of Haematology, 1979
- IRON ABSORPTION IN IRON-LOADING ANÆMIAS: EFFECT OF SUBCUTANEOUS DESFERRIOXAMINE INFUSIONSThe Lancet, 1977
- THE ROLE OF ERYTHROPOIETIN IN REGULATION OF POPULATION SIZE AND CELL CYCLING OF EARLY AND LATE ERYTHROID PRECURSORS IN MOUSE BONE MARROWCell Proliferation, 1977
- Erythroid colony formation in cultures of mouse and human bone marrow: Analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose‐concanavalin AJournal of Cellular Physiology, 1974
- EFFECT OF POLYCYTHEMIC SERUM ON THE RATE OF RED CELL PRODUCTION*Annals of the New York Academy of Sciences, 1968
- THE ROLE OF THE ERYTHROPOIETIC HORMONE IN ANIEMIAThe Lancet, 1961
- Biological Assay of Human Urinary Erythropoietic Stimulating Factor (ESF)Experimental Biology and Medicine, 1960
- Humoral Regulation of Erythropoiesis V. Relationship of Plasma Erythropoietine Level to Bone Marrow ActivityExperimental Biology and Medicine, 1959