RESOLUTION OF PHAGE-INITIATED AND NORMAL HOST THYMIDYLATE SYNTHETASES OF ESCHERICHIA COLI
- 1 February 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 48 (2), 242-247
- https://doi.org/10.1073/pnas.48.2.242
Abstract
Phage-initiated thymidylate synthetase has been separated on DEAE-cellulose columns from the corresponding normal host enzyme of E. coli. Uninfected cells showed only one thymidylate synthetase fraction. Infected cells showed two dTMP synthetases, one corresponding to the normal host enzyme. Both of these enzymes have been rechromatographed on standardized columns and shown to correspond in elution pattern to the original separations. After infection, E. coli 15[long dash][long dash][long dash]-exhibited only one dTMP synthetase, and this corresponded to the position on the DEAE-cellulose column of the phage-initiated enzyme.Keywords
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