Ultraviolet Surface Plasmon-Coupled Emission Using Thin Aluminum Films
- 8 June 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (14), 4076-4081
- https://doi.org/10.1021/ac040004c
Abstract
Surface plasmon-coupled emission (SPCE) is the directional radiation of light into a substrate due to excited fluorophores above a thin metal film. To date, SPCE has only been observed with visible wavelengths using silver or gold films. We now show that SPCE can be observed in the ultraviolet region of the spectrum using thin (20 nm) aluminum films. We observed directional emission in a quartz substrate from the DNA base analogue 2-aminopurine (2-AP). The SPCE radiation occurs within a narrow angle at 59° from the normal to the hemicylindrical prism. The excitation conditions precluded the creation of surface plasmons by the incident light. The directional emission at 59° is almost completely p-polarized, consistent with its origin from surface plasmons due to coupling of excited 2-AP with the aluminum. The emission spectra and lifetimes of the SPCE are those characteristic of 2-AP. Different emission wavelengths radiate at slightly different angles on the prism providing intrinsic spectral resolution from the aluminum film. These results indicate that SPCE can be used with numerous UV-absorbing fluorophores, suggesting biochemical applications with simultaneous surface plasmon resonance and SPCE binding assays.Keywords
This publication has 18 references indexed in Scilit:
- Multi-wavelength immunoassays using surface plasmon-coupled emissionBiochemical and Biophysical Research Communications, 2004
- Sensing molecular properties by ATR-SPP Raman spectroscopy on electrochemically structured sensor chipsElectrochimica Acta, 2003
- Directional surface plasmon-coupled emission: a new method for high sensitivity detectionBiochemical and Biophysical Research Communications, 2003
- Near-field photonics: surface plasmon polaritons and localized surface plasmonsJournal of Optics A: Pure and Applied Optics, 2003
- Single Molecule Imaging of Fluorescently Labeled Proteins on Metal by Surface Plasmons in Aqueous SolutionPhysical Review Letters, 1998
- A commercial solution for surface plasmon sensingSensors and Actuators B: Chemical, 1996
- Principles of biosensing with an extended coupling matrix and surface plasmon resonanceSensors and Actuators B: Chemical, 1993
- Choice of metal and wavelength for surface-plasmon resonance sensors: some considerationsApplied Optics, 1992
- Electromagnetic interactions of molecules with metal surfacesPhysics Reports, 1984
- Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation dataBiophysical Journal, 1984