Zur unterschiedlichen Infektiosität von isolierter Ribonucleinsäure und intaktem Poliovirus in der Gewebekultur

Abstract

Tissue culture cells in monolayers do not exchange measurable amounts of cellulare ribonuclease with the surrounding medium under conditions used for assay of biological active ribonucleic acid. The low degree of infectivity of isolated ribonucleic acid from poliovirus is, therefore, not caused by degradation of the ribonucleic acid by cellulare ribonuclease. Not all potentially infectious ribonucleic acid is taken up by the cells under standard assay conditions. Residual infectivity of ribonucleic acid in the supernatant of cell monolayers after incubation is considerably enhanced by a restoration of the hypertonicity of the solution.