Differential responsiveness of myc- and ras-transfected cells to growth factors: selective stimulation of myc-transfected cells by epidermal growth factor.
Open Access
- 1 March 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (3), 870-877
- https://doi.org/10.1128/mcb.6.3.870
Abstract
To identify functional relationships between oncogenes and growth factors, we compared the effects of transfected myc and ras oncogenes on the responsiveness of Fischer rat 3T3 cells to three growth factors: epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and transforming growth factor-beta (TGF-beta). Control cells did not grow in soft agar under any conditions. ras-Transfected cells grew in soft agar under all conditions tested and were insensitive to the stimulatory effects of exogenous growth factors. These cells secreted elevated levels of both EGF-like factors and TGF-beta, suggesting that the lack of responsiveness of these cells to exogenous growth factors arose from autocrine stimulation. myc-Transfected cells displayed conditional anchorage-independent growth: they formed numerous colonies in soft agar in the presence of EGF but relatively few colonies in the presence of PDGF or TGF-beta. Secretion of EGF-like factors and TGF-beta by these cells was not elevated above that of control cells. These results suggest a model for the mechanism of cooperation between myc and ras oncogenes in which ras-like genes induce growth factor production, while myc-like genes increase the responsiveness of cells to these factors.This publication has 57 references indexed in Scilit:
- The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF 1Cell, 1985
- Transcription Control by OncogenesCell, 1985
- Induction of c-fos gene and protein by growth factors precedes activation of c-mycNature, 1984
- Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and proteinNature, 1984
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cellsNature, 1984
- Nuclear location of the putative transforming protein of avian myelocytomatosis virusCell, 1982
- Properties of a sarcoma‐growth‐factor‐like peptide from cells transformed by a temperature‐sensitive sarcoma virusJournal of Cellular Physiology, 1981
- Autocrine Secretion and Malignant Transformation of CellsNew England Journal of Medicine, 1980
- Kirsten murine sarcoma virus transformed cell lines and a spontaneously transformed rat cell‐line produce transforming factorsJournal of Cellular Physiology, 1980