A high-frequency transformation system for the yeast Kluyveromyces lactis
- 1 November 1982
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 6 (2), 123-128
- https://doi.org/10.1007/bf00435211
Abstract
This communication describes conditions for Kluyveromyces lactis protoplast regeneration and transformation and shows that a high frequency of transformation can be obtained with recombinant plasmids containing one of a series of K. lactis DNA fragments that presumably carry autonomously replicating sequences (called KARS). The vector YRp7, which contains a Saccharomyces cerevisiae autonomously replicating sequence (ARS), only led to integrative transformation of K. lactis indicating substantial differences in specificity between the DNA replication mechanisms of both yeast species. It is further shown that 2-μm DNA derived vectors giving high frequency transformation in S. cerevisiae can transform K. lactis only with low frequency.Keywords
This publication has 19 references indexed in Scilit:
- Curing of Saccharomyces cerevisiae 2-?m DNA by transformationCurrent Genetics, 1981
- Isolation of a yeast centromere and construction of functional small circular chromosomesNature, 1980
- Expression of a transposable antibiotic resistance element in SaccharomycesNature, 1980
- Replication and recombination functions associated with the yeast plasmid, 2μ circleCell, 1980
- Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 geneGene, 1980
- Properties of Yeast TransformationCold Spring Harbor Symposia on Quantitative Biology, 1979
- Transformation of yeast by a replicating hybrid plasmidNature, 1978
- Molecular cloning and expression in E. coli of a yeast gene coding for β-galactosidaseCell, 1978
- Synthesis of high molecular weight polypeptides in Escherichia coli minicells directed by cloned Saccharomyces cerevisiae 2-μm DNAGene, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975