A simple method for the isolation of fraction I protein of chloroplasts
- 1 May 1968
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 46 (5), 441-444
- https://doi.org/10.1139/o68-066
Abstract
Application of the standard methods for the preparation of fraction I protein (ribulosediphosphate carboxylase) from spinach leaves gives low yields when applied to other species. Simple methods are described here for the preparation of pure fraction I protein in good yield from spinach, peas, beans, and Chlorella. The electrophoretic mobilities (on polyacrylamide electrophoretograms) of bean and Chlorella fraction I differ from each other and from the mobilities of pea and spinach protein.This publication has 4 references indexed in Scilit:
- Some physical properties of purified fraction I protein from spinach chloroplastsArchives of Biochemistry and Biophysics, 1967
- Spinach Ribulose Diphosphate Carboxylase. I. Purification and Properties of the Enzyme*Biochemistry, 1966
- An Improved Method for the Isolation of Carboxydismutase. Probable Identity with Fraction I Protein and the Protein Moiety of Protochlorophyll Holochrome*Biochemistry, 1965
- Antibiotic Substances from Cells and Culture Solutions of Unicellular Algae with Special Reference to some Chlorophyll DerivativesPhysiologia Plantarum, 1962