Über die Glykogenolyse im Warmblütermuskelbrei

Abstract

Guinea pig muscle was cut into small pieces with the scissors and 5 g. portions weighed into 200 cc. test tubes. Guinea pig muscle rich in glycogen was produced by injecting 12.5% glucose soln. into the guinea pigs at least 6 hrs. before death. The muscle samples were suspended in 25 cc. M/25 phosphate buffer, pH 7, and the tubes evacuated with the H2O pump. O2 was admitted to the tubes when desired. The tubes were shaken in the H2O bath at 25[degree]. Glycogen of the muscle decreased during incubation in O2 at 25[degree]. There was no direct relationship between decrease in glycogen and lactic acid formation in glycogen rich muscles. The total carbohydrate also remained unchanged. The glycogen appeared to be changed into carbohydrates of lower molecular wt. In O2 no glycogenolysis took place in beef muscle hash, which may have been due to lack of amylase, such as is present in guinea pig muscle. Neither vit. B1 nor insulin influenced the aerobic glycogenolysis of guinea pig muscle. Malonic acid increased glycogenolysis but the addition of most C4-dicarboxylic acids was without effect.