Human Renal Cell Carcinoma: Establishment and Characterization of Two New Cell Lines

Abstract

Characterization studies have been carried out on 2 cell lines (KPK 1 and KPK 13) established from human renal adenocarcinoma. KPK 1 and KPK 13 were passaged 178 times in vitro for about 6 yr and 7 mo. and 78 times for about 3 yr and 2 mo., respectively. Although morphologic differences exist between the 2 lines, each has an epithelial morphology and exhibits multilayering. Doubling time of KPK 1 and KPK 13 cells was 29 hours and 51 hours, respectively. Both KPK 1 and KPK 13 induced tumors at the site of s.c. injection, closely resembling the original tumor from which they were derived. Chromosome number of both cell lines was 100% aneuploid and the presence of Y chromosome was confirmed by G banding in KPK 13 cells. KPK 1 had high thromboplastic and high fibrinolytic activities, whereas KPK 13 had comparatively low thromboplastic and no detectable fibrinolytic activities. These activities were detected in the serum free supernatant fraction from KPK 1 cells but were not detected in that from KPK 13 cells.