Genetico-biochemical Analysis on the Enzyme-activities in the House Fly by Agar Gel Electrophoresis

Abstract

Thirteen distinct bands were exhibited in esterase activities in house flies. The E5, E6, E7, E11, and E13 esterase bands were not found in a diazinon resistant RP strain, while the E2, E8, E9, and E10 esterase bands were not detected in a susceptible and multichromosomal mutant strain, ro; ct; cm (2; 4; 5=rough eyed; cut wing; carmine eyed). All these esterases were found in zymograms of their hybrids. Comparisons of zymograms of each phenotype in the F2-progeny obtained from the backcross, (ro; ct; cm female X RP male) male, were carried out. The difference in esterase activities between the two strains was clearly segregated in the progeny: the E2, E5, E6, E7, E8, E9, or E13 bands (Eg, E7 and Eg, Eg bands were not easy to separate) might each be controlled by genetic factors on the 5th chromosome on which the eye color mutant cm gene and the diazinon resistant gene are located. The Amy-2 and Amy-4 bands were not detected in a multichromosal mutant strain, bwb; ocra; ct (2; 3; 4=brown-body; ocra eyed; cut wing), but the Amy-1 and Amy-3 were more active than those in RP strain. These all amylase activities were found in the RP strain and their hybrids. By comparing zymograms of each phenotype in the F2-progeny obtained from the backcross, bwb; ocra; ct female X F1 (bwb; ocra; ct female X RP male) male, it was assumed the Amy-2 and Amy-4 amylase activities might be controlled by genetic factor(s) on the 3rd chromosome on which the eye color mutant ocra gene is located.