Determining the Genetic Structure of the Natural Population of Staphylococcus aureus : a Comparison of Multilocus Sequence Typing with Pulsed-Field Gel Electrophoresis, Randomly Amplified Polymorphic DNA Analysis, and Phage Typing
Open Access
- 1 December 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (12), 4544-4546
- https://doi.org/10.1128/jcm.40.12.4544-4546.2002
Abstract
We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.Keywords
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