Contribution to the structural characterization of eucaryotic PSI reaction centre ? II. Characterization of a highly purified photoactive SDS-CP1 complex
- 1 January 1980
- journal article
- Published by Springer Nature in Photosynthesis Research
- Vol. 1 (1), 17-27
- https://doi.org/10.1007/bf00020072
Abstract
Under precise conditions, SDS PAGE† allows purification of a photoactive P700-chla-protein complex from eucaryotic cells. The yield of P700 recovery is close to 100%. A total protein content equivalent to about 140 kD for one mole of P700 has been estimated by chemical analysis, and electrophoresis revealed the presence of two peptidic chains with MWs close to 65 kD. Photochemical and structural properties of this complex are given and compared with those of other complexes previously isolated.Keywords
This publication has 31 references indexed in Scilit:
- A rapid procedure for isolating the photosystem i reaction center in a highly enriched formFEBS Letters, 1978
- Rapidly reversible flash‐induced electron transfer in a P‐700 chlorophyll—protein complex isolated with SDSFEBS Letters, 1978
- Heterogeneite des variations spectrales photoinduites vers 700 nm observees sur les membranes chlorophylliennes et les complexes chlorophylle-proteines isoles de divers organismes photosynthetiquesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- A critical evaluation of the analysis of membrane proteins by polyacrylamide gel electrophoresis in the presence of dodecyl sulphateJournal of Theoretical Biology, 1976
- A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii.The Journal of cell biology, 1975
- The molecular organization of chloroplast thylakoidsBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1975
- On the molecular nature of chloroplast thylakoid membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Bacterial PhotosynthesisAnnual Review of Microbiology, 1974
- The relationship between chlorophyll-protein complexes and chloroplast membrane polypeptidesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1974
- Purification of photosystem I reaction centers from spinach stroma lamellaeBiochemical and Biophysical Research Communications, 1970