Monoclonal Antibodies Directed Against Human la Antigens Detect an Evolutionary Conserved Epitope on Guinea Pig la Antigens With Unique Functional Properties
- 1 January 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Leukocyte Biology
- Vol. 35 (1), 101-113
- https://doi.org/10.1002/jlb.35.1.101
Abstract
Three murine monoclonal antihuman Ia antibodies were found to cross react with guinea pig Ia antigens. Sequential immunoprecipitation studies demonstrated that these antibodies recognized a subset of guinea pig Ia antigens. In competition binding studies, the antihuman Ia antibodies competed with each other, but not with a number of murine monoclonals raised directly to guinea pig Ia antigens. When the antihuman Ia monoclonals were tested for their ability to inhibit the proliferative response of primed guinea pig T cells to antigen-pulsed macrophages, they completely inhibited the response of strain 2 T cells to the complex antigen ovalbumin while having no effect on the response to the copolymer L-glutamic acid, L-lysine. In contrast, the monoclonals raised against guinea pig Ia had much less dramatic effects on the response of uncloned T cells to these antigens. These results suggest that the cross-reactive monoclonals may recognize an evolutionary conserved epitope on Ia that has a regulatory function in T-cell activation.This publication has 9 references indexed in Scilit:
- Analysis of HLA-DR antigens by using monoclonal antibodies: recognition of conformational differences in biosynthetic intermediates.The Journal of Immunology, 1981
- Antigen-specific T cell clones restricted to unique F(1) major histocompatibility complex determinants. Inhibition of proliferation with a monoclonal anti-Ia antibodyThe Journal of Experimental Medicine, 1981
- Monoclonal antibodies to guinea pig Ia antigens. I. Production, serologic, and immunochemical characterization.The Journal of Immunology, 1981
- Monoclonal antibody against an Ir gene product?The Journal of Experimental Medicine, 1980
- Monoclonal antibodies to guinea pig Ia antigens. II. Effect on alloantigen-, antigen-, and mitogen-induced T lymphocyte proliferation in vitro.The Journal of Experimental Medicine, 1980
- Two-gene control of the expression of a murine Ia antigen.The Journal of Experimental Medicine, 1978
- Labeling Characteristics and Separation of Ia Antigen SubunitsThe Journal of Immunology, 1978
- The guinea pig I region. I. A structural and genetic analysisThe Journal of Experimental Medicine, 1977
- Sharing of Ia antigens between species. I. Detection of Ia specificities shared by rats and mice.The Journal of Experimental Medicine, 1977