The Use of Protein A, from Staphylococcus aureus, in Immune Electron Microscopy for Detecting Plant Virus Particles
- 1 November 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 45 (2), 533-536
- https://doi.org/10.1099/0022-1317-45-2-533
Abstract
An immune electron microscopic technique for detecting plant viruses is described which involves pre-coating electron microscope grids with protein A (a wall protein of Staphylococcus aureus) before coating them with specific anti-serum. The method trapped 339 and 51 times more sugarcane mosaic virus and tobacco mosaic virus particles, respectively, than untreated grids. It appears particularly suitable for virus particles occurring in plant extracts in small numbers.This publication has 1 reference indexed in Scilit: