Treatment of type 1 diabetes mellitus in non-obese diabetic mice by transplantation of allogeneic bone marrow and pancreatic tissue.
- 1 September 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (18), 6555-6557
- https://doi.org/10.1073/pnas.84.18.6555
Abstract
Non-obese diabetic (NOD) mice provide a model for type 1 diabetes mellitus. We previously showed that allogeneic bone marrow transplantation (ABMT) can prevent and treat insulitis and overt diabetes in NOD mice. However, ABMT alone could not be used to treat overt diabetes in NOD mice whose islets had been completely destroyed. To provide insulin-producing cells, pancreatic tissue from newborn mice was grafted under the renal capsules in combination with ABMT. The aims of concomitant ABMT are as follows. (i) It induces immunological tolerance to the donor-type major histocompatibility complex determinants and permits the host to accept subsequent pancreatic allografts from the bone marrow donor. (ii) ABMT replaces abnormal stem cells with normal stem cells. After transplantation of bone marrow plus newborn pancreas, NOD mice showed reduction of the glycosuria and a normal response in the glucose-tolerance test. Immunohistological study revealed the presence of clustered insulin-containing beta cells in the grafted pancreatic transplants. ABMT may become a viable treatment of established type 1 diabetes mellitus in humans.This publication has 10 references indexed in Scilit:
- Successful liver allografts in mice by combination with allogeneic bone marrow transplantation.Proceedings of the National Academy of Sciences, 1986
- Prevention of type I diabetes in nonobese diabetic mice by allogenic bone marrow transplantation.Proceedings of the National Academy of Sciences, 1985
- Immunology of Insulin-Dependent Diabetes MellitusAnnual Review of Immunology, 1985
- Rationale for bone marrow transplantation in the treatment of autoimmune diseases.Proceedings of the National Academy of Sciences, 1985
- Molecular biology of Type 1 (insulin-dependent) diabetes mellitusDiabetologia, 1985
- Adoptive transfer of autoimmune diabetes mellitus in biobreeding/Worcester (BB/W) inbred and hybrid rats.The Journal of Immunology, 1985
- Passive Transfer of Diabetes in the BB/W RatScience, 1983
- Breeding of a Non-Obese, Diabetic Strain of MiceExperimental Animals, 1980
- Use of Polyethylene Glycol to Separate Free and Antibody-Bound Peptide Hormones in Radioimmunoassays†Journal of Clinical Endocrinology & Metabolism, 1971
- SIMULTANEOUS LOCALIZATION OF MULTIPLE TISSUE ANTIGENS USING THE PEROXIDASE-LABELED ANTIBODY METHOD: A STUDY ON PITUITARY GLANDS OF THE RATJournal of Histochemistry & Cytochemistry, 1968