Bioassay of Histamine in Human Urine: An Improved Method for Purification of Samples by Means of Cation Exchange Chromatography
- 1 January 1970
- journal article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 25 (4), 325-328
- https://doi.org/10.3109/00365517009046212
Abstract
Bioassay of histamine on the guinea-pig ileum was performed after puri fication of human urine by means of ion exchange columns prepared from either Dowex-50 or Amberlite IRC-50. The Dowex technique yielded an extract better suited for bioassay than the Amberlite technique. The dif ference was probably due to a lower content of interfering substances in the former extract. Values for the urinary excretion of histamine in healthy subjects are presented.Keywords
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