The action of certain drugs on the uptake of 32P by human erythrocytes

Abstract

The uptake of inorganic radioactive phosphate by fresh human erythrocytes has been observed over a period of 60 minutes. Cell-phosphate fractions have been separated by paper chromatography and the total phosphate and P32 contents of 4 fractions were estimated: (a) 2,3-diphospho-glycerate and fructose 1,6-diphosphate; (b) adenosine triphosphate; (c) inorganic phosphate; (d) hexose monophosphate. Adenosine triphosphate was more heavily labeled than the other fractions at 15 and 60 minutes after the addition of tracer. Sodium thioquinalbarbitone (0.25 m[image]), sodium thiopentone (0.25 m[image]), sodium quinalbarbitone (0.25 m[image]), sodium pentobarbitone (1.0 m[image]), sodium hexobarbitone (2.0 m), phenylbutazone (2.0 m[image]), 2,4-dinitrophenol (0.5 m[image]), iodoacetate (0.5 m[image]) and chloroform (0.02[image]) reduced the phosphate exchange. Sodium salicylate and sodium thiopentone depressed the p32 uptake of 2,3-diphosphoglycerate and adenosine triphosphate to a greater extent than they depressed the P32 exchange of inorganic phosphate and hexose monophosphate. These agents do not depress p32 uptake by inhibiting triose phosphate dehydrogenase, for neither drug had any effect on glycolysis. The effects of 2,4-dinitrophenol and phenylbutazone resembled those of sodium salicylate and sodium thiopentone on the P32 uptake of the phosphate fractions.