Humanization of an anti-p185HER2 antibody for human cancer therapy.

Abstract

Summary 1.As part of a study on alternative pathways of glucose metabolism in tumor tissues in vivo, a single intraperitoneal injection of 4–8 mg. of glucose-1-C¹⁴ containing 10 μc. of radioactivity (11 × 10⁶ c.p.m.) was given to rats bearing 10-day-old Flexner-Jobling carcinomas, and the rats were studied over a period of 1–18 hours. 2.During this period 56 per cent of the injected C¹⁴ had appeared in the breath as CO₂, with a sharp decrease in the rate of output between 2 and 3 hours. 3.The ribonucleic acid (RNA) of the tumors contained 2,000 cpm/gm of tissue at 1 hour and 4,380 c.p.m. at 5 hours, and the C¹⁴ was almost entirely in the ribose moiety. The corresponding values for the livers from the same animals were 0 and 534, respectively. 4.The desoxyribonucleic acid (DNA) of the tumors contained 650 and 2,260 cpm/gm at 1 and 5 hours, respectively, while the DNA of the liver did not incorporate significant levels of C¹⁴ at any time. 5.The acid-soluble fractions of liver and tumor were shown to contain 48,500 and 56,100 cpm/gm of tissue, respectively, at 1 hour, and 9,500 and 6,500 c.p.m. at 5 hours. Much of this C¹⁴ was present in forms other than glucose. 6.Concomitant studies on the incorporation of glucose C¹⁴ into protein showed that the maximum occurred at about 5 hours in both liver and tumor, which then contained 32 and 65 per cent, respectively, of the decrease in acid-soluble radioactivity that occurred between 1 and 5 hours. 7.Several additional tissues from the tumor-bearing animals were studied in less detail.