Detection of Sprouted Wheat by a Rapid Colorimetric Determination of α-Amylase

Abstract

A method is described for the rapid, convenient detection or estimation of sprout-damaged wheat by determining its α-amylase content colorimctrically. A commercially available substrate in the form of dyed amylose tablets is used. The extent of sprout damage can be determined qualitatively, or quantitatively when actual α-amylase concentration is required. Amylase content is determined by visual comparison with prepared colored standards or by spectrophotometric analysis. The method requires only 5 min incubation time and no elaborate equipment, and is sufficiently simple for use at locations having minimal laboratory facilities.