Detection of Sprouted Wheat by a Rapid Colorimetric Determination of α-Amylase
Open Access
- 1 January 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 60 (1), 16-20
- https://doi.org/10.1093/jaoac/60.1.16
Abstract
A method is described for the rapid, convenient detection or estimation of sprout-damaged wheat by determining its α-amylase content colorimctrically. A commercially available substrate in the form of dyed amylose tablets is used. The extent of sprout damage can be determined qualitatively, or quantitatively when actual α-amylase concentration is required. Amylase content is determined by visual comparison with prepared colored standards or by spectrophotometric analysis. The method requires only 5 min incubation time and no elaborate equipment, and is sufficiently simple for use at locations having minimal laboratory facilities.This publication has 1 reference indexed in Scilit:
- A new method for the determination ofα-amylaseCellular and Molecular Life Sciences, 1967