Ultrastructural Changes and Biochemical Events in Basidiospore Germination of Schizophyllum commune

Abstract

Electron microscopic features and biochemical events were outlined in basidiospore germination of Schizophyllum commune. Normal ultrastructural changes included prominent vacuolization and more abundant endoplasmic reticulum. A lag phase in outgrowth included depletion of cellular reserves of trehalose, mannitol, and arabitol and subsequent increases in ribonucleic acid and protein. Depletion of polyols required exogenous carbon and nitrogen sources and was arrested by protein synthesis antagonists. Outgrowth subsequent to the lag period was accompanied by increased glycogen deposition and alkali-soluble glucan production.