Albumin testing in urine using a smart-phone
- 9 August 2013
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 13 (21), 4231-4238
- https://doi.org/10.1039/c3lc50785h
Abstract
We demonstrate a digital sensing platform, termed Albumin Tester, running on a smart-phone that images and automatically analyses fluorescent assays confined within disposable test tubes for sensitive and specific detection of albumin in urine. This light-weight and compact Albumin Tester attachment, weighing approximately 148 grams, is mechanically installed on the existing camera unit of a smart-phone, where test and control tubes are inserted from the side and are excited by a battery powered laser diode. This excitation beam, after probing the sample of interest located within the test tube, interacts with the control tube, and the resulting fluorescent emission is collected perpendicular to the direction of the excitation, where the cellphone camera captures the images of the fluorescent tubes through the use of an external plastic lens that is inserted between the sample and the camera lens. The acquired fluorescent images of the sample and control tubes are digitally processed within one second through an Android application running on the same cellphone for quantification of albumin concentration in the urine specimen of interest. Using a simple sample preparation approach which takes ∼5 min per test (including the incubation time), we experimentally confirmed the detection limit of our sensing platform as 5–10 μg mL−1 (which is more than 3 times lower than the clinically accepted normal range) in buffer as well as urine samples. This automated albumin testing tool running on a smart-phone could be useful for early diagnosis of kidney disease or for monitoring of chronic patients, especially those suffering from diabetes, hypertension, and/or cardiovascular diseases.Keywords
This publication has 57 references indexed in Scilit:
- Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopyNature Methods, 2012
- Integrated rapid-diagnostic-test reader platform on a cellphoneLab on a Chip, 2012
- Quantum dot enabled detection of Escherichia coli using a cell-phoneThe Analyst, 2012
- The contribution of chronic kidney disease to the global burden of major noncommunicable diseasesKidney International, 2011
- Optofluidic Fluorescent Imaging Cytometry on a Cell PhoneAnalytical Chemistry, 2011
- Cost-effective and compact wide-field fluorescent imaging on a cell-phoneLab on a Chip, 2010
- Lensfree microscopy on a cellphoneLab on a Chip, 2010
- Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applicationsLab on a Chip, 2010
- Chronic Kidney Disease and Its ComplicationsPrimary Care: Clinics in Office Practice, 2008
- Prevalence of Chronic Kidney Disease in the United StatesJAMA, 2007