A Modified, High Yield Procedure for the Synthesis of Unlabeled and 14C-Labeled 4-Methylene-DL-glutamic Acid
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry & Biotechnology
- Vol. 11 (3), 339-350
- https://doi.org/10.1080/00327488108061773
Abstract
This paper describes the complete chemical synthesis of 4-methylene-DL-glutamic acid from diethylmalonate, formaldehyde and diethyl acetamidomalonate. The amino acid was obtained pure following ion-exchange chromatography and/or crystallization from hot water in an overall yield of 30% based on the amount of diethylmalonate used. Several physico-chemical characteristics of the synthetic compound were determined, including ir and pmr spectra, chromatography on paper, retention time on an amino acid analyzer, pK values and melting point; all properties cf the synthetic material were found to be identical to those seen with the naturally occurring L-isomer. The procedure for obtaining gram quantities of the unlabeled compound has also been modified for the synthesis of high specific activity (10.6 mCi/nmol) 4-methylene-[2-14C]-DL-glutamic acid.This publication has 4 references indexed in Scilit:
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- C14O2 ASSIMILATION IN LILIUM REGALE WITH REFERENCE TO GAMMA-METHYLENEGLUTAMIC ACID1956
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