Fusion of protoplasts of Bacillus megaterium.
- 1 June 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (6), 2147-2150
- https://doi.org/10.1073/pnas.73.6.2147
Abstract
Protoplasts of 2 doubly auxotrophic strains of B. megaterium were fused by nascent calcium phosphate or by polyethylene glycol treatment and then allowed to revert to bacillary form on selection media. The colonies formed were analyzed and were mixed populations of individual bacteria apparently containing parental, recombinant and segregating genotypes. The evidence suggested that segregation was transitory, lasting only for a few clonings and reisolations.This publication has 7 references indexed in Scilit:
- Fusion of bacterial protoplasts.Proceedings of the National Academy of Sciences, 1976
- Fusion of fungal protoplastsNature, 1974
- The Effect of High pH and Calcium on Tobacco Leaf Protoplast FusionZeitschrift für Naturforschung C, 1973
- Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different SpeciesNature, 1965
- THE ISOLATION OF PROTOPLASTS FROM BACILLUS MEGATERIUM BY CONTROLLED TREATMENT WITH LYSOZYMEJournal of Bacteriology, 1953
- TRANSFER OF PENICILLIN RESISTANCE IN PNEUMOCOCCI BY THE DESOXYRIBONUCLEATE DERIVED FROM RESISTANT CULTURESCold Spring Harbor Symposia on Quantitative Biology, 1951