Independent expression of type i collagen and fibronectin by normal fibroblast-like cells
Open Access
- 1 September 1983
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 63 (1), 233-244
- https://doi.org/10.1242/jcs.63.1.233
Abstract
A double-label immunofluorescence technique was used to determine whether all normal fibroblast-like cells cultured from porcine connective tissues simultaneously express both type I collagen and fibronectin (FN). Cells surrounding Explants in primary cultures from gingival and periodontal ligament (PL) tissue were heterogeneous in the expression of collagen and FN. Mass population cells at first and second subculture after primary explanting of gingival tissue were also heterogeneous in the expression of collagen and FN. When clonal populations were analysed, four distinct phenotypes were observed : Coll+/FN+; C0II+/FN–; Coll–/FN+; Coll–/FN–. Mass populations of PL cells were more homogeneous than gingival fibroblasts and when clones were analysed only two phenotypes were observed. These data suggest that normal fibroblast-like cells in vitro are capable of expressing collagen and FN independently and that FN-negative cells may utilize a FN-independent attachment mechanism in culture.This publication has 32 references indexed in Scilit:
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