Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (19), 7843-7853
- https://doi.org/10.1093/nar/17.19.7843
Abstract
Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M. kansasii was determined and found to be 98.7% homologous to that of M. bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.Keywords
This publication has 26 references indexed in Scilit:
- Rapid ribosomal RNA sequencing and the phylogenetic analysis of protistsParasitology Today, 1989
- Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single CellsScience, 1989
- Polymerase chain reaction reveals cloning artefactsNature, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Compilation of small ribosomal subunit RNA sequencesNucleic Acids Research, 1988
- Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNANature, 1987
- HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitusNature, 1987
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology, 1987
- Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic SequencesScience, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985