Complexity of control of tumorigenic expression in intraspecies hybrids of human SV40-transformed fibroblasts and normal human fibroblast cell lines
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 35 (4), 263-268
- https://doi.org/10.1159/000131878
Abstract
Somatic cell hybrids between SV40-transformed human fibroblast cells and normal human fibroblasts were isolated and tested for growth in vitro and in athymic nujnu (nude) mice. All hybrid cells formed by a cross of WI-18/VA-2, a tumorigenic SV40-transformed human fibroblast, with IMR-90 expressed T antigen and grew in the appropriate selective medium. However, tumorigenic potential ranged from completely suppressed to fully expressed in these hybrid cells. In addition, all hybrid cells formed between LNSV, a nontumorigenic SV40-transformed human fibroblast, and IMR-90 were nontumorigenic. Previous studies have shown that the tumorigenic potential of hybrids cells formed between a variety of human tumor cell lines and normal human fibroblasts is completely suppressed. These results imply that the genetic control of tumorigenic expression in virally transformed human cells differs from that seen in spontaneously arising human tumor cells.Keywords
This publication has 3 references indexed in Scilit:
- Genetic analysis of tumorigenesis: IV. chromosome reduction and marker segregation in progeny clones from Chinese hamster cell hybridsSomatic Cell and Molecular Genetics, 1979
- Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice.Proceedings of the National Academy of Sciences, 1977
- Morphological and virological investigation of human tissue cultures transformed with SV40Journal of Cellular and Comparative Physiology, 1963