Plasma Secretin Concentration and Pancreatic Exocrine Secretion after Intravenous Secretin or Intraduodenal HCL in Anaesthetized Pigs
- 1 May 1977
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Gastroenterology
- Vol. 12 (3), 267-272
- https://doi.org/10.3109/00365527709180927
Abstract
The concentration of immunoreactive secretin in arterial blood and the exocrine pancreatic secretion were measured during intraportal infusion of secretin in doses of 0.03, 0.06, 0.1, 0.3, 1.0 and 3.0 clinical units kg-1 h-1, and during intraduodenal instillation of 50 ml 0.1 mol 1-1 HCl. The lowest dose of exogenous secretin to significantly increase secretin concentration in blood was 0.03 clinical units kg-1 h-1, which was a subthreshold dose of exocrine pancreatic secretion. A linear relation was found between the dose of secretin and the secretin concentrations measured. On the basis of secretin concentrations, release of secretin during instillation of HCl was estimated to be 0.22 clinical units kg-1 h-1. Maximum pancreatic bicarbonate secretion was obtained with a dose of 1.0 clinical units kg-1 h-1, and the minimal effective dose was between 0.06 and 0.1 clinical units kg-1 h-1. On the basis of the flow rate of pancreatic juice and the pancreatic bicarbonate output, release of secretin during instillation of HCl was estimated to be 0.3 clinical unit kg-1 h-1. It is concluded that the radioimmunoassay used has the sensitivity and accuracy necessary for measurements of secretin concentrations in plasma during physiological conditions.Keywords
This publication has 11 references indexed in Scilit:
- Production and Evaluation of Antibodies for Radioimmunoassay of SecretinScandinavian Journal of Clinical and Laboratory Investigation, 1976
- Comparison of vasoactive intestinal peptide and secretin in stimulation of pancreatic secretion.The Journal of Physiology, 1976
- Preparation of125I-labelled Synthetic Porcine Secretin for RadioimmunoassayScandinavian Journal of Clinical and Laboratory Investigation, 1976
- Alcohol-Stimulated Secretion of Immunoreactive SecretinNew England Journal of Medicine, 1975
- The role of secretin in the inhibition of gastric secretion by intraduodenal acidGut, 1974
- Effects of Intraduodenal Administration of HCl and Glucose on Circulating Immunoreactive Secretin and Insulin ConcentrationsJCI Insight, 1974
- Studies on the Elimination of Secretin and Cholecystokinin with Regard to the Kinetics of Exocrine Pancreatic SecretionDigestion, 1974
- Measurement of Endogenous Release of Cholecystokinin by RadioimmunoassayAnnals of Surgery, 1973
- Pancreatic response to acidification of various lengths of proximal intestine in the dogAmerican Journal of Physiology-Legacy Content, 1970
- The Interaction of Secretin and Pancreozymin on the External Pancreatic Secretion in DogsActa Physiologica Scandinavica, 1967