MAJOR PROTEINS OF ESCHERICHIA-COLI OUTER CELL-ENVELOPE MEMBRANE SEQUENCE OF CYANOGEN-BROMIDE FRAGMENTS OF PROTEIN-I FROM ESCHERICHIA-COLI B-R
- 1 January 1978
- journal article
- research article
- Vol. 359 (12), 1807-1810
Abstract
The sequence of the CNBr fragments of 1 of the major outer membrane proteins of E. coli B/r was established with the aim of elucidating the primary structure of this protein. Separation of all fragments on 1 molecular sieve column was achieved upon citraconylation of these fragments. Overlapping peptides were obtained by digestion of the protein, or a CNBr fragment arising from incomplete cleavage, with trypsin or Staphylococcus aureus protease.This publication has 5 references indexed in Scilit:
- Primary structure of the membranous segment of cytochrome b5.Proceedings of the National Academy of Sciences, 1977
- Major proteins of the outer cell envelope membrane ofEscherichia coli K-12: Multiple species of protein IMolecular Genetics and Genomics, 1977
- Pleiotropic transport mutants of Escherichia coli lack porin, a major outer membrane proteinMolecular Genetics and Genomics, 1977
- Identification of the outer membrane protein of E.coli that produces transmembrane channels in reconstituted vesicle membranesBiochemical and Biophysical Research Communications, 1976
- The Sequence Determination of a Protein in a Micro Scale: The Sequence Analysis of Ribosomal Protein L34 ofEscherichia coliHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976