Isolation of an acute hypotensive substance from bovine brain lipid fraction.

Abstract

A depressor substance was found in acetone extract of bovine brain, greatly purified with the use of various chromatographic techniques, and tentatively named as D-I. It caused a sharp fall in arterial blood pressure of anesthetized cats with i.v. injections of 10 .mu.g level of purified sample per kg animal body weight. Its hypotensive activity was not affected by previous treatment of cats with atropine, diphenhydramine or hexamethonium. In the enzymic assay with the use of prostaglandin dehydrogenase, neither prostaglandin E [PGE] nor PGF compound was found. In the test of an incubation with a proteinase (nagase), the depressor activity was recovered almost completely. This depressor substance is definitely distinguishable from other depressor compounds and relatively similar to lysolecithin, in the comparison of its attitude on TLC or in the course of purification process.