Spectrophotometry Determination of Carboxylic Acids by Ferric Hydroxamate Formation with Water-Soluble Carbodiimide
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 13 (7), 603-609
- https://doi.org/10.1080/00032718008077690
Abstract
Carboxylic acids in aqueous solution were determined spectrophotometrically as ferric hydroxamates. A water-soluble carbodiimide, l-cyclohexyl-3-(2-morpho-linyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate, was used as a cuopling agent to form hydroxamic acids. The coupling reaction was carried out at 37°C for 30 min in a solution buffered at pH 5.3 with pyridine and hydrochloric acid. Calibration curves of benzoic, acetic, citric, and a-ketoglutaric acids were linear between 0.25 and 8 μmol.Keywords
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