Temporal Analysis of the Antigenic Composition of Borrelia burgdorferi during Infection in Rabbit Skin
Open Access
- 1 September 2004
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (9), 5063-5072
- https://doi.org/10.1128/iai.72.9.5063-5072.2004
Abstract
The numbers of host-adapted Borrelia burgdorferi (HAB) organisms in rabbit skin were assessed by real-time PCR over the first 3 weeks of infection. Maximal numbers were found at day 11, while spirochete numbers decreased by more than 30-fold by day 21. The antigenic composition of HAB in skin biopsy samples was determined by use of a procedure termed hydrophobic antigen tissue Triton extraction. Immune sera from rabbits, sera from chronically infected mice, and monospecific antiserum to the antigenic variation protein, VlsE, were used to probe parallel two-dimensional immunoblots representing each time point. Individual proteins were identified using either specific antisera or by matching protein spots to mass spectrometry-identified protein spots from in vitro-cultivated Borrelia. There were significant changes in the relative expression of a variety of known and previously unrecognized HAB antigens during the 21-day period. OspC and the outer membrane proteins OspA and OspB were prominent at the earliest time point, day 5, when the antigenic variation protein VlsE was barely detected. OspA and OspB were not detected after day 5. OspC was not detected after day 9. VlsE was the most prominent antigen from day 7 through day 21. BmpA, ErpN, ErpP, LA7, OppA-2, DbpA, and an unidentified 15-kDa protein were also detected from day 7 through day 21. Immunoblot analysis using monospecific anti-VlsE revealed the presence of prominent distinct VlsE lower forms in HAB at days 9, 11, and 14; however, these lower forms were no longer detected at day 21. This marked diminution in VlsE lower forms paralleled the clearance of the spirochete from skin.Keywords
This publication has 65 references indexed in Scilit:
- Immunohistochemical Analysis of Lyme Disease in the Skin of Naive and Infection-Immune Rabbits following ChallengeInfection and Immunity, 2001
- Homologous and Heterologous Borrelia burgdorferi Challenge of Infection-Derived Immune Rabbits Using Host-Adapted OrganismsInfection and Immunity, 2001
- Comparison of Protection in Rabbits against Host-Adapted and CultivatedBorrelia burgdorferifollowing Infection-Derived Immunity or Immunization with Outer Membrane Vesicles or Outer Surface Protein AInfection and Immunity, 2000
- New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresisElectrophoresis, 1998
- Acquired resistance to Borrelia burgdorferi infection in the rabbit. Comparison between outer surface protein A vaccine- and infection-derived immunity.Journal of Clinical Investigation, 1997
- Immunolocalization of a 22 kDa protein (IPLA7, P22) ofBorrelia burgdorferiFEMS Microbiology Letters, 1996
- Immunolocalization of a 22 kDa protein ( IPLA7, P22) of Borrelia burgdorferiFEMS Microbiology Letters, 1996
- Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.Journal of Clinical Investigation, 1995
- Nucleotide sequence and analysis of the gene inBorrelia burgdorferiencoding the immunogenic P39 antigenFEMS Microbiology Letters, 1994
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970