Quantitative Determination of Individual Serum Proteins by Radio-electroimmuno and Use of125I-Labelled Antibodies (Application to C-reactive Protein)

Abstract

The sensitivity of quantitative electrophoresis into agarose gel containing antibodies was increased about 60-fold by incorporation of radioactive antibodies in the precipitates. The precipitates were visualised by autoradiography. The technique was exemplified with the use of rabbit-anti-CRP-gammaglobulin-¹²⁵I in the quantification of CRP. The method, which can measure the proteins down to a concentration of 0.01 mg/1, invariably demonstrated CRP in apparently healthy blooddonors.