Chloroplast TatC plays a direct role in thylakoid ΔpH‐dependent protein transport
- 10 July 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 501 (1), 65-68
- https://doi.org/10.1016/s0014-5793(01)02626-6
Abstract
The thylakoid (Delta)pH-dependent pathway transports folded proteins. Identified components include Hcf106 and Tha4. Orthologs of these proteins plus a membrane protein called TatC are essential for the homologous bacterial Tat system. Here we report identification of a chloroplast TatC (cpTatC). cpTatC is an integral thylakoid membrane protein as determined by in vitro chloroplast import and immunoblotting. Antibody to cpTatC specifically inhibited the thylakoid (Delta)pH-dependent pathway in vitro. cpTatC is present in about the same quantity as estimated translocation sites, whereas Hcf106 and Tha4 are present in 5-8-fold excess. These results are relevant to mechanistic models for this system.Keywords
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