Interaction of target cell-bound C3bi and C3d with human lymphocyte receptors. Enhancement of antibody-mediated cellular cytotoxicity.
Open Access
- 1 June 1981
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 153 (6), 1592-1603
- https://doi.org/10.1084/jem.153.6.1592
Abstract
The occurrence and distribution of distinct receptors for 3 C3 [complement component 3] fragments on purified human blood lymphocytes was studied by rosette formation. Indicator cells were bovine, chicken or sheep erythrocytes (E) bearing up to 100,000 molecules of human C3b (EC3b) without antibody. EC3b was converted to C3bi-bearing-E (EC3bi) with purified C3b inactivator (factor I) and .beta.1H (factor H), and to c3d-bearing E (EC3d) by treatment of EC3bi with trypsin. Using bovine E (Eb) as indicators, .apprx. 11% of the lymphocytes bound EbC3b, 6% bound EbC3bi and 2% bound EbC3d. Fractionation of the lymphocytes by adsorption to monolayers of C3-fragment-bearing Eb or by rosetting indicated that most of the cells with receptors for C3b were distinct from those having receptors for C3bi and/or C3d. Cells from 2 lymphoblastoid cell lines ([human Burkitt''s lymphoma] Raji and Daudi) formed strong rosettes with EC3bi or EC3d. A fraction of the Raji cells, but not of Daudi cells formed rosettes with EC3b, which were weak. 51Cr-labeled E was used as a target in antibody-dependent, lymphocyte-mediated cytotoxicity (ADCC). In the absence of antibody, not lysed by the lymphocytes. At sub optimal concentrations of IgG anti-E antibody, ADCC of C3-fragment-bearing E was strongly enhanced. The enhancing capacity of the fragments occurred in the order of C3bi > C3d .mchgt. C3b. C3-fragment-bearing cells inhibited the lysis of antibody-coated cells not bearing C3 fragments. Inhibition ranked in the same order as enhancement. Target cell bound C3 fragments apparently enhance ADCC by improving contact between target cells and those effector cells which have C3 receptors. Cell-bound C3 fragments inhibit ADCC of C3-free targets by impeding their contact with such effector cells. Certain lymphocytes probably are capable of interacting with C3bi in addition to C3b and C3d; C3bi and C3d probably have a greater regulatory effect on their cytolytic function than C3b.This publication has 25 references indexed in Scilit:
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