Preparation of Highly Potent Human Pituitary Gonadotrophins.

Abstract
Summary Human FSH and LH fractions, equal in potency to any previously reported, have been further purified by gel filtration and ion-exchange chromatography. The most potent of these new fractions was 94 times more active than NIH-FSH-S1 and 2.9 times more active than NIH-LH-S1, respectively. The writers are indebted to Jean Batchelder, Lynn Crook, and Karin Westphal for skilled technical assistance, to Professors G. A. Brecher and A. E. Wilhelmi for critical reading of the manuscript, and to Endocrinology Study Section, N.I.H., for supplies of XIH-LH-S1 and NIH-FSH-S1.