The Effect of Inhibition of (ADP‐ribose)n Biosynthesis on DNA Repair Assayed by the Nucleoid Technique
Open Access
- 1 December 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 121 (1), 65-69
- https://doi.org/10.1111/j.1432-1033.1981.tb06430.x
Abstract
DNA damage and repair was assayed by the loss and restoration of DNA supercoiling in nucleoids. This technique was used to assess the effects of inhibition of (ADP-ribose)n biosynthesis by 3-aminobenzamide on the capacity to repair DNA of mouse leukaemia L1210 cells following damage by γ-irradiation and by the mono- functional alkylating agent, dimethyl sulphate. 3-Aminobenzamide partially inhibits restoration of supercoiling following γ-irradiation and dimethyl sulphate treatment, but inhibits neither the enzymic incision events leading to breaks in the DNA nor the repair synthesis. This inhibition of repair can be observed at very low doses of damaging agents. These observations confirm and extend the evidence that (ADP-ribose)n biosynthesis is required for efficient cellular recovery from DNA damage. In particular, the nucleoid technique permits the demonstration that 3-aminobenzamide inhibits DNA repair after γ-radiation; it is not possible to draw this unequivocal conclusion with the data from alkaline sucrose gradients because this technique is too insensitive. 1-β-D-Arabinofuranosylcytosine, which inhibits seiniconservative DNA replication, also retards repair, and this effect can be reversed by the addition of deoxycytidine. The inhibitors of DNA excision repair, arabinosyl- cytosine, hydroxyurea or 3-aminobenzoamide increase the steady-state number of DNA breaks. Thus, they can be used to enhance even further the sensitivity of the nucleoid assay of repair.This publication has 14 references indexed in Scilit:
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