Virtual 2-D Gel Electrophoresis: Visualization and Analysis of the E. coli Proteome by Mass Spectrometry
- 31 July 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 73 (17), 4063-4070
- https://doi.org/10.1021/ac0101858
Abstract
Mass spectrometric surface analysis of isoelectric focusing gels provides an ultrasensitive approach to proteome analysis. This “virtual 2-D gel” approach, in which mass spectrometry is substituted for the size-based separation of SDS−PAGE, provides advantages in mass resolution and accuracy over classical 2-D gels and can be readily automated. Protein identities can be postulated from molecular mass (±0.1−0.2% for proteins of 2 to E. coli proteins.This publication has 29 references indexed in Scilit:
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