Ouabain-binding-site photoaffinity probes that label both subunits of Na+,K+-ATPase.
- 1 August 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (8), 4529-4533
- https://doi.org/10.1073/pnas.77.8.4529
Abstract
4"''-Diazomalonyldigitoxin and its isomer, 3"''-diazomalonyldigitoxin, were synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of enzyme with both photolabels showed ouabain-protectable labeling of the .alpha. and .beta. subunit. These data suggest that, in the purified eel enzyme, the .alpha. and .beta. subunits are in intimate contact, at least in the region of the 3rd digitoxose of the sugar-specific binding site.This publication has 18 references indexed in Scilit:
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